Simulation of the systematic outcome of a reaction.
The simulation starts from 50 000 initial target copies and = 1.95. The results are shown in various representations: fluorescence versus cycle (panel A), ln2() versus fluorescence (panel B), and efficiency versus cycle (panel C). The dashed line represents self limiting conditions, the solid line represents primer depleting conditions. The vertical dotted line in panel A represents the phase switch criterion: . The two arrows in panel C mark the position of the second derivative maxima, the arrows in panel B mark the corresponding positions in the efficiency vs. cycle plot.