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Sildenafil induces in vitro angiogenesis, DRG neurite outgrowth and Schwann cell migration.

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posted on 2015-02-17, 03:21 authored by Lei Wang, Michael Chopp, Alexandra Szalad, LongFei Jia, XueRong Lu, Mei Lu, Li Zhang, Yi Zhang, RuiLan Zhang, Zheng Gang Zhang

Representative microscopic images (A to D) and quantitative data (I) show capillary-like tube formation of endothelial cells under regular glucose (RG), high glucose (HG), high glucose with sildenafil (HG+sil, 300 nM), and high glucose with sildenafil in the presence of a neutralizing antibody against Tie2 (+Tie2, 5 μg/ml). Panels E to H show NFH immunoreactive DRG neuron cultured in conditional medium derived from endothelial cells treated with regular glucose (RG), high glucose (HG), high glucose with sildenafil (HG+sil, 300 nM), and high glucose with sildenafil in the presence of a neutralizing antibody against Tie2 (+Tie2, 5 μg/ml). Panels J and K show quantitative data of neurite outgrowth from DRG neurons and the percentage of Schwann cell migration under different conditions listed above. Panel L shows ELISA data of Ang1 levels in conditional medium harvested from endothelial cells cultured with regular glucose (RG), high glucose (HG), high glucose with sildenafil (+sil, 300 nM). *p<0.05 versus regular glucose (RG), and #p<0.05 versus high glucose (HG), respectively. n = 6/group. Bar in D = 50 μm and Bar in H = 20 μm.

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