Figure_2.tif (5.59 MB)

Sik3^−/− mice are resistant to a high-fat diet.

figure

posted on 2012-05-25, 00:51 authored by Tatsuya Uebi, Yumi Itoh, Osamu Hatano, Ayako Kumagai, Masato Sanosaka, Tsutomu Sasaki, Satoru Sasagawa, Junko Doi, Keita Tatsumi, Kuniko Mitamura, Eiichi Morii, Katsuyuki Aozasa, Tomohiro Kawamura, Meinoshin Okumura, Jun Nakae, Hajime Takikawa, Toshio Fukusato, Minako Koura, Mayumi Nish, Anders Hamsten, Angela Silveira, Alejandro M. Bertorello, Kazuo Kitagawa, Yasuo Nagaoka, Hidehisa Kawahara, Takeshi Tomonaga, Tetsuji Naka, Shigeo Ikegawa, Noriyuki Tsumaki, Junichiro Matsuda, Hiroshi Takemori

(A) A representative image of male mice after high-fat (60% of calories) feeding. Mice (n = 6) were fed with a high-fat diet for 12–30 weeks and their body weights were monitored every week (B). Means and SEM are shown. All data points indicate p<0.001. (C) Representative photos of tissues (scale, 1 mm). (D) Tissue weights are shown. *, **, and *** indicate p<0.05, <0.01, and <0.001, respectively. (E) Levels of blood glucose, insulin, and leptin measured after 4-h fasting. (F) Glucose tolerance test (1.5 g/kg) performed after 4-h fasting. (G) Cholesterol and triglyceride content in feces (n = 3 cages). (H) Oxygen consumption (VO₂, voluntarily O₂ consumption) of each group (n = 6) was monitored. Respiration quotient (RQ) during the day and night. (I) Cholesterol (Chol) and triglycerides (TG) in the liver and serum were measured (n = 6). (J) Serum Chol and TG were separated using FPLC.