Setdb2 expression by Mϕ influences T cell proliferation in IAV-infected lungs.
(A, B) Control and Setdb2ffLyz2cre+ mice infected with 1 x 104 PFU of IAV and euthanized on days 6, 8, and 10 post-infection. (A) The number of CD4+ and CD8+ T cells in IAV-infected lungs. (B) To measure proliferation, mice were injected with the thymidine analogue EdU one day prior to euthanization. The number of proliferating (EdU+) CD4+ and CD8+ T cells was calculated over the indicated time course. Values represent mean ± SEM (n = 5–20 mice) from 2–4 independent experiments. *p<0.05; **p<0.01; ***p<0.001 (relative to control lungs).