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Sequential images of C. subdepressus embryos during gastrulation.

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posted on 2010-03-22, 02:25 authored by Bruno C. Vellutini, Alvaro E. Migotto

Vegetal pole is bottom. A Post-hatching swimming blastula with thickened vegetal pole epithelium 9 hpf. B Initial ingression of PMC into the blastocoel 10 hpf. C Ingressed PMC aggregated on the posterior end 11 hpf. D Initial migration of PMC through the inner side of ectoderm 13 hpf; red-pigmented cells are present on the vegetal pole (not visible). E Initial invagination of the archenteron 14 hpf. F SMC ingress the blastocoel from the archenteron tip 15 hpf; PMC form lateral aggregates (arrows) and begin to secrete the skeleton. G SMC reaching the anterior pole 16.5 hpf (arrow); red-pigmented cells reached the middle region of the embryo through the ectoderm. H Final stage of archenteron invagination with SMC touching the anterior pole 19 hpf; blastocoel is populated with PMC and SMC and red-pigmented cells reached the anterior pole. Scale bars = 

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