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Sensitivity of the Total DnaK to the Change in the Transcription Rate Constant for DnaK at Low Temperature

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posted on 2013-02-22, 08:35 authored by Hiroyuki Kurata, Hana El-Samad, Rei Iwasaki, Hisao Ohtake, John C Doyle, Irina Grigorova, Carol A Gross, Mustafa Khammash

The plot reports the sensitivity computed for the parameters values that satisfy the required yield and efficiency (yield > 0.9 and efficiency > 0.85 at low and high temperature). Yield and efficiency were computed by searching the 2-D parameter space consisting of the transcription rate constant for σ32 and the association constant between σ32 and DnaK as follows:

for the various systems,

(A) DEG-FB/FF double mutant (SEQ-FB system): K[5] = K[6] = 0 M−1, kx[1] = kx[2] = 0 min−1, η = 1,

(B) FF mutant (SEQ-FB + DEG-FB system): K[5] = K[6] = 1 × 108 M−1, kx[1] = kx[2] = 5 min−1, η = 1,

(C) Wild-type (SEQ-FB +DEG-FB + FF system): K[5] = K[6] = 1 × 108 M−1, kx[1] = kx[2] = 5 min−1, η = 1 (low), η = 2 (high).

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