Senescence of detached leaves from WS and PLDδ-KO plants.

Leaves detached from WS and PLDδ-KO plants were treated with sterile water or 50 µM ABA for 5 days. Retardation of ABA-promoted senescence was compared between PLDδ-KO and WS leaves. A. Yellow coloration (top panel) or low Fv/Fm values for variable fluorescence (bottom panel) indicated the degree of senescence. The color bar at the bottom indicates Fv/Fm values. B. Effects of exogenous ABA on the chlorophyll content of leaves from WS and PLDδ-KO plants. FW, fresh weight. Values are means ± SDs (n = 5). C. Effects of exogenous ABA on cell death in leaves from WS and PLDδ-KO plants. Cell death was determined spectrophotometrically using Evan’s blue staining. Values are means ± SDs (n = 5). Values with different letters are significantly different (p<0.05). “*” indicates that the value is significantly different from that for WS under the same condition (p<0.05).