Figure_3.tif (1.65 MB)
Selected RNA-aptamer AK48 binds to Blom7α
figure
posted on 2012-10-29, 00:40 authored by Marlies Löscher, Markus Schosserer, Eric Dausse, Kiseok Lee, Paul Ajuh, Regina Grillari-Voglauer, Angus I. Lamond, Jean-Jacques Toulmé, Johannes Grillari. (A) Consensus sequence of putative binding motif of all selected sequences (Blom7α wt and Blom7-KH) in alignment with AK48 (red: putative binding motif, underscore: AC-rich part and poly-C stretch. (B, C) Result of SPR analysis using AK48 (0.68 µM) and non/weak binding KH-specific RNAs (0.74 µM) on immobilized Blom7α wt (B) or Blom7-KH (C). (D, E) Result of SPR analysis using AK48 (0.68 and 2 µM) on immobilized Blom7α wt (D) or Blom7-KH (E).
