Screening of transgenic cassava lines for resistance to Sri Lankan cassava mosaic virus.
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posted on 2015-04-22, 03:37 authored by Valentine Otang Ntui, Kynet Kong, Raham Sher Khan, Tomoko Igawa, Gnanaguru Janaky Janavi, Ramalingam Rabindran, Ikuo Nakamura, Masahiro MiiFour week-old hardened transgenic and wild-type plants lines were mechanically inoculated twice with SLCMV. Symptom development was assessed every alternate day and photographs were taken at 7, 14 and 28 days post inoculation (dpi). WT, non-transformed wild-type plant. K3, susceptible transgenic line. K4 resistant transgenic line. Arrow indicates leaf deformation.
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RNA interferenceSri Lankan Cassava Mosaic VirusKU 50agronomic performancePCR amplificationvirus loadSLCMVKU 50 linesRNA Silencing Cassava ranksTransgenic linesbioethanol productionGenetically Engineered Cassava cvgrowth abnormalityengineer resistanceincrease biomassdnainoculated control plantsAV 1AV 2High Level Expression
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