Screening for CMV specific T cells with three different Class I-tetramers.

PBMC from 4 healthy CMV pp65 responding donor were stimulated for 8 day with a mixture of 15 amino acid long overlapping peptides spanning the entire pp65 protein. The cells were analyzed pp65 specific responses using the IC-IFNγ assay and stained for CD8, CD69 and intracellular IFNγ (top panel). The donors were screened for HLA-A*0101 restricted pp65_363–373, the HLA-A*0201 restricted pp65_495–503, and the HLA-B*0702 restricted pp65_417–426 specific T cells. Staining the cells with CD8 and each of the three tetramers, HLA-A*0101-pp65_363–373, HLA-A*0201-pp65_495–503, and HLA-B*0702-pp65_417–426. The dot plot shows the gated CD8 T cells. Numbers in the upper right quadrant of each plot are the percentage of epitope specific CD8 T cells.