Figure_2.tif (143.76 kB)
Scheme of construction of NYVAC-C-KC-ΔB19R.
figure
posted on 2013-02-20, 11:48 authored by Karen V. Kibler, Carmen E. Gomez, Beatriz Perdiguero, Shukmei Wong, Trung Huynh, Susan Holechek, William Arndt, Victoria Jimenez, Ruben Gonzalez-Sanz, Karen Denzler, Elias K. Haddad, Ralf Wagner, Rafick P. Sékaly, James Tartaglia, Giuseppe Pantaleo, Bertram L. Jacobs, Mariano EstebanTo insert C7L and K1L back into the NYVAC genome, each gene plus a corresponding portion of the flanking regions was amplified by PCR. The two fragments were combined into one fragment using PCR. The entire cassette, containing C7L and K1L plus flanking regions homologous to the adjacent genes of the NYVAC genome (C8L and K2L), was inserted into NYVAC-C by in vivo recombination. To create NYVAC-C-KC-ΔB19R, the KC fragment was inserted into NYVAC-C-ΔB19R by the same method. IGR: Intergenic region.
