Schematic vector maps of the silencing constructs used for transformation.

Anti-sense constructs: Dde fragments of 198 or 523 bps were cloned in anti-sense orientation downstream of the fcpA promoter (pDDE-AS198 and pDDE-AS523). Inverted repeat constructs: Dde fragments of 293 and 523 bp lengths were cloned in sense and anti-sense orientation. The two fragments were linked with an eGFP fragment supposed to function as spacer. Amp: Ampicillin resistance; Zeo: Zeocin™ resistance; fcpA: Fucoxanthin Chlorophyll a/c-binding Protein A promoter; eGFP: enhanced green fluorescent protein.