Schematic presentation of the <i>phaCAB</i> operon of <i>R. eutropha</i> PHB<sup>-</sup>4.

<p>Primers <i>phaCAB_fw</i> and <i>phaCAB_rv</i> were applied to generate the 4,860-bp DNA fragment comprising the <i>phaCAB</i> operon. The PCR fragment was subcloned into vector pJET1.2/blunt, and subsequently fragments of three independent hybrid plasmids were sequenced. The arrow marks the observed G320A mutation in <i>phaC</i> causing a stop codon obviously leading to a truncated and non functional PHA synthase (PhaC) in strain PHB<sup>-</sup>4.</p>