Schematic presentation of the phaCAB operon of R. eutropha PHB^-4.

Primers phaCAB_fw and phaCAB_rv were applied to generate the 4,860-bp DNA fragment comprising the phaCAB operon. The PCR fragment was subcloned into vector pJET1.2/blunt, and subsequently fragments of three independent hybrid plasmids were sequenced. The arrow marks the observed G320A mutation in phaC causing a stop codon obviously leading to a truncated and non functional PHA synthase (PhaC) in strain PHB^-4.