Figure_1.tif (720.66 kB)
Schematic of Adherent Cell-SELEX (AC-SELEX) Procedure.
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posted on 2013-02-20, 06:11 authored by Jessica C. Graham, Helmut ZarblA starting library of >1015 aptamers was utilized and eighteen rounds of AC-SELEX were carried out to purify and amplify the aptamers which recognized epitopes present on HF cells and not present on HeLa cells. The starting library was first incubated with the HeLa cell line and the unbound aptamers were recovered and utilized for the AC-SELEX procedure.