Sch B inhibits TGF-β induced EMT of 4T1 and primary human breast cancer cells in vitro.

Cells were treated with Sch B (5 µg/ml) and TGF-β (5 ng/ml) as described in Materials and Methods. (A) Representative photos of TGF-β induced morphological change of 4T1 cells with or without pretreatment of Sch B. (B) Fluorescence immunostaining of cellular E-cadherin, vimentin, fibronectin, and F-actin. (C) Western blotting of E-cadherin, vimentin, Snail, Slug, ZEB1, with β-actin as the internal control. (D) and (E), TGF-β induced migration and invasion of 4T1 cells with or without pretreatment of Sch B. (F) Representative photos of TGF-induced the morphological change of primary breast cancer cells with or without pretreatment of Sch B and western blotting of E-cadherin and vimentin of primary breast cancer cells from three individual patients.