Sampling monomeric cooperativity of mammalian glucokinase.

2015-04-14T02:49:45Z (GMT) by Pedro Saa Lars K. Nielsen
<p>(A) Probability distribution of the Hill coefficient for the glucokinase. Approximately 93% out of 10<sup>4</sup> sampled kinetics displays cooperative behaviour. (B) Probability distribution for the ratio of the forward and reverse rate constants from the low- to the high-affinity enzyme state. The great majority (98.1%) of the models exhibiting positive cooperativity agrees with a slow transition from the low- to the high-affinity enzyme states. (C) Comparison of the kinetic space described by the model developed by Storer and Cornish-Bowden [<a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1004195#pcbi.1004195.ref048" target="_blank">48</a>] (blue) and the sampled kinetics using the mnemonic model (red). Each line represents the real kinetic behaviour described by the two models, while the shaded areas denote one-standard-deviation confidence regions for each approach.</p>