SST decreases Rac1 activity in migrating keratinocytes.

A: The amount of active Rac1 was determined by affinity precipitation with purified GST-PAK[PBD]-fusion protein from keratinocyte lysates 3 h after induction of migration. SST treatment reduces active Rac1 compared to untreated cells. Precipitation with GST alone was used as negative control. (I) input, (P) precipitate. B: Relative quantification of Rac1 activity (control = 100%; n = 4). Results are shown as means+/−SEM, ** P<0.01.