Figure_2.tif (390.86 kB)

SR-uPA^+/0 macrophages are polarized to adopt M2 phenotype.

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posted on 2013-03-12, 09:02 authored by Jessica Meznarich, Laura Malchodi, Deri Helterline, Stephen A. Ramsey, Kate Bertko, Tabitha Plummer, Abigail Plawman, Elizabeth Gold, April Stempien-Otero

A. Expression of M2 genes measured by qrtPCR in SR-uPA^+/0 and NTG macrophages. Columns represent the mean of arbitrary C units normalized to Gapdh, error bars are S.D., n = 5 mice per genotype. B. Increase in expression of markers of M2 activation in response to IL-4 (10 ng/ml) in SR-uPA^+/0 and NTG bone marrow derived macrophages. Columns represent means and error bars represent S.E.M. P values are calculated by Mann-Whitney as data were non-parametric, n = 5–6 mice per genotype. C. Production of TNF-alpha protein after serial treatment with IL-4 followed by LPS in SR-uPA^+/0 and NTG macrophages. Circles represent the mean of duplicate samples from individual mice, n = 3 mice per genotype. D. Fold increase in expression of TNF-alpha mRNA after treatment with LPS or LPS+IL-4 in SR-uPA^+/0 and NTG macrophages, n = 4 mice per genotype. E. Arginase activity in conditioned media from SR-uPA^+/0 and NTG macrophages. N = 4 mice per genotype F. Immunoblot for Arg1 in protein extract from SR-uPA^+/0 and NTG macrophages.