SOX-17 over-expression or BMP2-supplementation is sufficient to to resyore calcium transients and synchrony in HDAC1-KD ES cells.
A. Calcium uptake and release experiments indicating synchrony and response to stimuli of beating EBs in mES, mES-HDAC1-KD, mES-HDAC1-KD embryoid bodies treated with BMP2 for the first two days of differentiation. B. Percentage of EBs that respond to external stimuli and are synchronized (example shown in A). C. RT-PCR showing mRNA expression levels of Connexin-43 and D. organization of Connexin 43, in mES, mES-HDAC1-KD derived embryoid bodies and mES-HDAC1-KD embryoid bodies treated with BMP2 for the first two days of differentiation. Data in all panels are represented as mean +/− SD and n = 3.