SOX-17 over-expression or BMP2-supplementation is sufficient to to resyore calcium transients and synchrony in HDAC1-KD ES cells.

<p><b>A.</b> Calcium uptake and release experiments indicating synchrony and response to stimuli of beating EBs in mES, mES-HDAC1-KD, mES-HDAC1-KD embryoid bodies treated with BMP2 for the first two days of differentiation. <b>B.</b> Percentage of EBs that respond to external stimuli and are synchronized (example shown in A). <b>C.</b> RT-PCR showing mRNA expression levels of Connexin-43 and <b>D.</b> organization of Connexin 43, in mES, mES-HDAC1-KD derived embryoid bodies and mES-HDAC1-KD embryoid bodies treated with BMP2 for the first two days of differentiation. Data in all panels are represented as mean +/− SD and n = 3.</p>