Figure_3.tif (225.79 kB)
SDS-PAGE pattern of proteins associated with PBCV-1 DNA.
figure
posted on 2012-02-16, 00:19 authored by Timo Wulfmeyer, Christian Polzer, Gregor Hiepler, Kay Hamacher, Robert Shoeman, David D. Dunigan, James L. Van Etten, Marco Lolicato, Anna Moroni, Gerhard Thiel, Tobias MeckelDNA was released from capsids by osmotic shock and separated from soluble proteins by centrifugation. The DNA-containing pellet was treated with DNase to release DNA-bound proteins. The framed bands were excised and used for MALDI TOF analysis. Lane 1: weight marker, lane 2: proteins obtained after DNAse treatment.