SDS-PAGE gels of recombinant rotavirus proteins expressed by recombinant baculoviruses.
(I and II) Expressed recombinant VP4 with P and P genotypes, respectively. (III, IV and V) VP4 with P genotypes expressed by recombinant dualcistronic baculoviruses containing ORFs coding for VP4 and VP7. Expressed VP7 was not detected following staining with Coomasie brilliant blue. (VI) Recombinant VP2 and VP6 (lane 2) expressed by dualcistronic baculoviruses containing VP2 and VP6 encoding ORFs, and recombinant VP6 (lanes 3 and 4) expressed by monocistronic baculoviruses containing the VP6 encoding ORF. #1, #2, #3, #4 and #5 designate different plaques purified from the same construct on each gel. Lanes: Ladder, PageRuler Plus Prestain Protein Ladder (Fermentas UAB, Vilnius, Lithuania); Wild-type, empty baculovirus used as a control.