RvD1 drives polarization of alternatively activated macrophages.

Mice were exposed to air or CS with or without RvD1 as described. (A) Expression of Arg-1 was measured in whole lung homogenates by Western blotting. Each lane represents one animal. B–F: Alveolar macrophages were enriched from BAL cells by adherence and total RNA was extracted and subjected to quantitative RT-PCR to measure mRNA levels of Arg1 (B), Mrc1 (C), IL-10 (D), iNOS (E) and TNFα (F). Mean ± SEM is shown, normalized to 18S rRNA. Each group n = 6 for Arg1, Mrc1 and IL-10; n = 3 for iNOS and TNFα. ^#P<0.05, ^##P<0.01, compared to Vehicle/Air animal by one-way ANOVA with Bonferroni post-tests; *P<0.05, **P<0.01, ***P<0.001 versus Vehicle/Smoke animal by two-way ANOVA with Bonferroni post-test. ‡P<0.05 between RvD1/Air and RvD1/Smoke, ANOVA.