Rotavirus virus-like particles produced in insect cells by using dsRNA of wild-type strains.

<p>(<b>I</b>) dRV-VLPs produced by infecting High Five cells with recombinant baculoviruses containing ORFs coding for VP2 (C2) and VP6 (I2) proteins. Scale bar 200 nm. (<b>II</b>) tRV-VLPs produced by infecting Sf9 cells with recombinant baculoviruses containing ORFs coding for VP2 (C2), VP4 (P<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0105167#pone.0105167-Madhi1" target="_blank">[6]</a>), VP6 (I2) and VP7 (G9) proteins. Scale bar 200 nm. (<b>III</b>) Chimaeric tRV-VLPs produced by infecting High Five cells with recombinant baculoviruses containing ORFs coding for VP2 (C2), VP4 (P<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0105167#pone.0105167-Vesikari1" target="_blank">[4]</a>), VP6 (I2) and VP7 (G8) proteins. Scale bar 200 nm. (<b>IV</b>) Chimaeric tRV-VLPs produced by infecting Sf9 cells with recombinant baculoviruses containing ORFs coding for VP2 (C2), VP4 (P<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0105167#pone.0105167-Madhi1" target="_blank">[6]</a>), VP6 (I2) and VP7 (G2) proteins. Scale bar 200 nm. <b>V</b>) Chimaeric tRV-VLPs produced by infecting High Five cells with recombinant baculoviruses containing ORFs coding for VP2 (C2), VP4 (P<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0105167#pone.0105167-Glass1" target="_blank">[8]</a>), VP6 (I2) and VP7 (G8) proteins. Scale bar 200 nm. <b>VI</b>) Chimaeric tRV-VLPs produced by infecting High Five cells with recombinant baculoviruses containing ORFs coding for VP2 (C2), VP4 (P<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0105167#pone.0105167-Glass1" target="_blank">[8]</a>), VP6 (I2) and VP7 (G9) proteins. Scale bar 100 nm. <b>VII</b>) Chimaeric tRV-VLPs produced by infecting Sf9 cells with recombinant baculoviruses containing ORFs coding for VP2 (C2), VP4 (P<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0105167#pone.0105167-Vesikari1" target="_blank">[4]</a>), VP6 (I2) and VP7 (G12) proteins. Scale bar 100 nm. <b>VIII</b>) Chimaeric tRV-VLPs produced by infecting High Five cells with recombinant baculoviruses containing ORFs coding for VP2 (C2), VP4 (P<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0105167#pone.0105167-Vesikari1" target="_blank">[4]</a>) and VP6 (I2) proteins. Scale bar 200 nm. tRV-VLPs with a smooth outer ring are shown with arrows.</p>