Rotavirus virus-like particles produced in insect cells by using dsRNA of wild-type strains.

(I) dRV-VLPs produced by infecting High Five cells with recombinant baculoviruses containing ORFs coding for VP2 (C2) and VP6 (I2) proteins. Scale bar 200 nm. (II) tRV-VLPs produced by infecting Sf9 cells with recombinant baculoviruses containing ORFs coding for VP2 (C2), VP4 (P[6]), VP6 (I2) and VP7 (G9) proteins. Scale bar 200 nm. (III) Chimaeric tRV-VLPs produced by infecting High Five cells with recombinant baculoviruses containing ORFs coding for VP2 (C2), VP4 (P[4]), VP6 (I2) and VP7 (G8) proteins. Scale bar 200 nm. (IV) Chimaeric tRV-VLPs produced by infecting Sf9 cells with recombinant baculoviruses containing ORFs coding for VP2 (C2), VP4 (P[6]), VP6 (I2) and VP7 (G2) proteins. Scale bar 200 nm. V) Chimaeric tRV-VLPs produced by infecting High Five cells with recombinant baculoviruses containing ORFs coding for VP2 (C2), VP4 (P[8]), VP6 (I2) and VP7 (G8) proteins. Scale bar 200 nm. VI) Chimaeric tRV-VLPs produced by infecting High Five cells with recombinant baculoviruses containing ORFs coding for VP2 (C2), VP4 (P[8]), VP6 (I2) and VP7 (G9) proteins. Scale bar 100 nm. VII) Chimaeric tRV-VLPs produced by infecting Sf9 cells with recombinant baculoviruses containing ORFs coding for VP2 (C2), VP4 (P[4]), VP6 (I2) and VP7 (G12) proteins. Scale bar 100 nm. VIII) Chimaeric tRV-VLPs produced by infecting High Five cells with recombinant baculoviruses containing ORFs coding for VP2 (C2), VP4 (P[4]) and VP6 (I2) proteins. Scale bar 200 nm. tRV-VLPs with a smooth outer ring are shown with arrows.