Rho kinase is activated in peripheral blood cells after 30-min ischemia and 12-h reperfusion (I/R).

<p>The mRNA expression of Rho kinase 1 (A) and 2 (B) in leukocytes 12 h after I/R was determined by quantitative RT-PCR. Expression levels of MYPT-1 and phosphorylated MYPT-1 were determined by immunoblotting (C). Results of the quantitative analysis of phosphorylated MYPT-1 are shown (D). Data represent means from at least 5 mice each. * p<0.05, ** p<0.01.</p>