Restricted feeding induces c-fos expression in the DMH and LHA at ZT4.
A. Correlation of ZT4 DMH c-fos densitometry values with number of c-fos puncta. B. Quantitation of ZT4 DMH c-fos mRNA. Bar graphs represent c-fos expression levels normalized to the WT-Ad Lib average value, expressed as means ±S.E.M. White bars indicate ad libitum feeding conditions, black bars indicate restricted feeding conditions. B. Correlation of locomotor activity to DMH c-fos gene expression. For correlation graphs, locomotor activity levels and c-fos expression levels are normalized to the WT-Ad Lib average values. C. Quantitation of ZT4 LHA c-fos mRNA. D. Correlation of locomotor activity to LHA c-fos gene expression.