Rescue of growth of the pmr1-Δ pmc1-Δ double mutant by CaCl2 and MnCl2.

Cell suspensions with equal densities were spotted onto corresponding media and grown for 2 days. The experiment was performed using serial dilutions of cell suspensions (S5 Fig) and a representative dilution is shown in this figure. PMR1 pmc1-Δ and pmr1-Δ pmc1-Δ, the 1MA77/12/GAP2-Δpmc strain with or without the PMR1-containing plasmid, respectively; PMR1 PMC1 and pmr1-Δ PMC1, the 1MA77/12/GAP2 strain with or without the PMR1-containing plasmid, respectively.