Representative images of the rat retina after rotenone exposure and the effect of rAAV5-NDI1.

<p>Microspheres loaded with rotenone was injected into the SC of rat brain. Two months later, the retina was examined for damage using hematoxylin-eosin staining. A, representative images of the retina; B, comparison of the cell layer thickness of the retina. The histogram shows the average of the thickness of the retina cell layers after hematoxylin-eosin staining (n = 4 for rats, n = 16 for retina slices from each rat retina). For both <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0011472#pone-0011472-g001" target="_blank">Figure 1A and 1B</a>, a, control; b, rAAV5-NDI1 injection only; c, rotenone microspheres injection only; d, rAAV5-NDI1 injected immediately after the rotenone-beads administration; e, rAAV5-NDI1 injected 1 week after the rotenone microspheres administration; f, rAAV5-NDI1 injected 2 weeks postadministration of rotenone microspheres; g, rAAV5-GFP injected immediately after the rotenone microspheres administration; h, rAAV5-empty vector injected immediately after the rotenone microspheres administration; i, rAAV5-GFP injected 2 weeks after the rotenone microspheres exposure; j, rAAV5-empty vector injected 2 weeks after the rotenone microspheres administration. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer. *p<0.05, **p<0.01, Student T-test compared to the same retina layer of control (a).</p>