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Representative examples of low and high AQUA scores.

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posted on 2014-10-01, 02:54 authored by Gloria B. Roldán Urgoiti, Karla Gustafson, Alexander C. Klimowicz, Stephanie K. Petrillo, Anthony M. Magliocco, Corinne M. Doll

4',6-diamidino-2-phenylindole (DAPI) defines the nuclear compartment, Alexa-555 defines pan-cytokeratin (PCK) positive anal carcinoma cells and the tumor cytosolic compartment. The “merge” panels are pseudo-colored red for p16, green for pan-cytokeratin (epithelial cell/tumor marker), and blue for DAPI (nuclear stain).

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