Representative examples of low and high AQUA scores.

<p>4',6-diamidino-2-phenylindole (DAPI) defines the nuclear compartment, Alexa-555 defines pan-cytokeratin (PCK) positive anal carcinoma cells and the tumor cytosolic compartment. The “merge” panels are pseudo-colored red for p16, green for pan-cytokeratin (epithelial cell/tumor marker), and blue for DAPI (nuclear stain).</p>