Replication of Huh-7 – passaged cloned DENV-2.

(a) Huh-7 – passaged sylvatic P8-1407 DENV-2 on Huh-7 cells. (b) Huh-7 – passaged endemic IQT-1950 DENV-2 on Huh-7 cells. (c) Huh-7 – passaged sylvatic P8-1407 DENV-2 on the bypassed cell line C6/36. (d) Huh-7 – passaged endemic IQT-1950 DENV-2 on the bypassed cell line C6/36. (e) Huh-7 – passaged sylvatic P8-1407 DENV-2 on a control cell line (Vero). (f) Huh-7 – passaged endemic IQT-1950 DENV-2 on a control cell line (Vero). Timepoint T = 0 represents residual virus after washing.