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Relaxation of supercoiled DNA.

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posted on 2013-07-02, 03:23 authored by Barbara Arnò, Ilda D’Annessa, Cinzia Tesauro, Laura Zuccaro, Alessio Ottaviani, Birgitta Knudsen, Paola Fiorani, Alessandro Desideri

Relaxation of negative supercoiled plasmid in a time course experiment for hTop1 and hTop1(pf-Linker) in presence of DMSO (lanes 1–9) and 100µM CPT (lanes 10–18); lane 19, no protein added. The reaction products are resolved in an agarose gel and visualized with ethidium bromide. The two forms of the supercoiled plasmid DNA are indicated as "Dimer" and "Monomer".

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