Relative quantification of L2 mRNA expression.

(A) Amplification plot based on quantitative real time PCR of HPV16 L2 expression. L2 is transcribed in E2 intact/episomal (episomal or concomitant) but in E2 disrupted/integrated cases. (B) Dissociation curve depicting the first-derivative melting curve for the reaction characterizing the expression of L2 (Tm of 80.5°C). (C) Amplification plot based on quantitative real time PCR of ACTB expression. ACTB is expressed by both episomal and integrated HPV16 positive cases. (D) Dissociation curve depicting the first-derivative melting curve for the reaction characterizing the expression of ACTB (Tm of 81.0°C).