Figure_4.tif (522.53 kB)
Regulation of the intra-mitochondrial content of ALA-mediated PpIX by Ko143, a specific inhibitor of ABCG2.
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posted on 2013-02-19, 19:45 authored by Hirotsugu Kobuchi, Koko Moriya, Tetsuya Ogino, Hirofumi Fujita, Keiji Inoue, Taro Shuin, Tatsuji Yasuda, Kozo Utsumi, Toshihiko UtsumiU937 cells and A549 cells were incubated for 3 h with 1 mM ALA in complete medium in the presence or absence of 1 µM Ko143. After incubation, cells were washed with ALA-free medium and mitochondrial and cytosolic fractions were isolated as described in Materials and Methods. The fluorescence spectrum of each fraction was analyzed by a fluorophotometer. A representative histogram of three independent experiments is shown. The levels of PpIX were shown by black line (No treatment), red line (ALA without Ko143), green line (ALA with Ko143), respectively.