Reduced mitochondrial activity in muscles expressing PABPN1-17ala.

<p>A) Quantification of mitochondrial DNA (mtDNA) content in control and PABPN1-17ala- expressing thoraxes at day 2, using qPCR. Three mitochondrial genes (<i>mt</i>:<i>CoI</i>, <i>mt</i>:<i>CoII</i> and <i>mt</i>:<i>cyt-b</i>) were analysed. Mitochondrial DNA levels were normalized to <i>RpL32</i> DNA. Means are from three biological replicates, error bars represent standard deviation. ns: not significant, using the Student’s t-Test. B) Activities of mitochondrial respiratory chain complexes were analysed by spectrophotometry from control and PABPN1-17ala-expressing thoraxes (genotypes as in A). Means are from five biological replicates, error bars represent standard deviation. * <i>p</i>-value <0.05, *** <i>p</i>-value <0.001, ns: not significant, using the Student’s t-Test. C) Quantification of mRNA levels of transcription factors regulating mitochondrial function in control and PABPN1-17ala-expressing thoraxes at day 2, using RT-qPCR (genotypes as in A). mRNA levels were normalized to <i>sop</i> mRNA. Means are from two biological replicates quantified three times, error bars represent standard deviation. *** <i>p</i>-value <0.001, ns: not significant, using the Student’s t-Test. D) Overexpression of <i>ewg</i> and <i>dERR</i> genes reduces the wing posture phenotypes of flies expressing PABPN1-17ala. Wing posture phenotypes were scored at day 6, at 18°C from OPMD (<i>w</i><sup><i>1118</i></sup><i>; UAS-PABPN1-17ala/+; Mhc-Gal4/+</i>), OPMD; <i>OE-spargel</i> (<i>w</i><sup><i>1118</i></sup><i>; UAS-PABPN1-17ala/+; Mhc-Gal4/</i>s<i>pargel</i><sup><i>EY05931</i></sup>), OPMD; <i>OE-delg</i> (<i>w</i><sup><i>1118</i></sup><i>; UAS-PABPN1-17ala</i>, <i>UAS-delg-HA/+; Mhc-Gal4/+</i>), OPMD; <i>OE-ewg</i> (<i>w</i>, <i>ewg</i><sup><i>EY05137</i></sup><i>/Y; UAS-PABPN1-17ala/+; Mhc-Gal4/+</i>) and OPMD; <i>OE-dERR</i> (<i>w</i><sup><i>1118</i></sup><i>; UAS-PABPN1-17ala/+; Mhc-Gal4/dERR</i><sup><i>G4389</i></sup>) flies (n > 130). *** <i>p</i>-value <0.001, using the χ<sup>2</sup> test.</p>