Fig_2.tif (712.78 kB)

Reduced mitochondrial activity in muscles expressing PABPN1-17ala.

figure

posted on 2015-03-27, 04:22 authored by Aymeric Chartier, Pierre Klein, Stéphanie Pierson, Nicolas Barbezier, Teresa Gidaro, François Casas, Steven Carberry, Paul Dowling, Laurie Maynadier, Maëlle Bellec, Martine Oloko, Claude Jardel, Bodo Moritz, George Dickson, Vincent Mouly, Kay Ohlendieck, Gillian Butler-Browne, Capucine Trollet, Martine Simonelig

A) Quantification of mitochondrial DNA (mtDNA) content in control and PABPN1-17ala- expressing thoraxes at day 2, using qPCR. Three mitochondrial genes (mt:CoI, mt:CoII and mt:cyt-b) were analysed. Mitochondrial DNA levels were normalized to RpL32 DNA. Means are from three biological replicates, error bars represent standard deviation. ns: not significant, using the Student’s t-Test. B) Activities of mitochondrial respiratory chain complexes were analysed by spectrophotometry from control and PABPN1-17ala-expressing thoraxes (genotypes as in A). Means are from five biological replicates, error bars represent standard deviation. * p-value <0.05, *** p-value <0.001, ns: not significant, using the Student’s t-Test. C) Quantification of mRNA levels of transcription factors regulating mitochondrial function in control and PABPN1-17ala-expressing thoraxes at day 2, using RT-qPCR (genotypes as in A). mRNA levels were normalized to sop mRNA. Means are from two biological replicates quantified three times, error bars represent standard deviation. *** p-value <0.001, ns: not significant, using the Student’s t-Test. D) Overexpression of ewg and dERR genes reduces the wing posture phenotypes of flies expressing PABPN1-17ala. Wing posture phenotypes were scored at day 6, at 18°C from OPMD (w¹¹¹⁸; UAS-PABPN1-17ala/+; Mhc-Gal4/+), OPMD; OE-spargel (w¹¹¹⁸; UAS-PABPN1-17ala/+; Mhc-Gal4/spargel^EY05931), OPMD; OE-delg (w¹¹¹⁸; UAS-PABPN1-17ala, UAS-delg-HA/+; Mhc-Gal4/+), OPMD; OE-ewg (w, ewg^EY05137/Y; UAS-PABPN1-17ala/+; Mhc-Gal4/+) and OPMD; OE-dERR (w¹¹¹⁸; UAS-PABPN1-17ala/+; Mhc-Gal4/dERR^G4389) flies (n > 130). *** p-value <0.001, using the χ² test.