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Reduced macrophage content in adipose tissue after inhibition of glycosphingolipid content with the iminosugar AMP-DNM.

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posted on 23.03.2009 by Marco van Eijk, Jan Aten, Nora Bijl, Roelof Ottenhoff, Cindy P. A. A. van Roomen, Peter F. Dubbelhuis, Ingar Seeman, Karen Ghauharali-van der Vlugt, Hermen S. Overkleeft, Cynthia Arbeeny, Albert K. Groen, Johannes M. F. G. Aerts

(A) Immunohistochemical analysis of crown-like structures in LepOb mice using double staining for F4/80 (red) and perilipin (blue). F4/80 staining in (B) LepOb and (C)AMP-DNM fed LepOb mice. (D) Quantification of crown-like structures in lean, LepOb and AMP-DNM treated LepOb mice. (E) Real time PCR analysis of F4/80 expression and (F) CD11c expression in lean, LepOb and LepOb mice fed AMP-DNM. Data are depicted on the Y-axis as mean±S.E.M. (n = 5 per group). Actual p values are depicted in the graphs. Bars in the photographs represent 100 µm.