Recruitment of IcmSW to the membrane by the <i>dotL</i> mutants.

<p>Subcellular localization of IcmS was assayed using <i>L. pneumophila</i> lysates separated into total (T), soluble (S) and membrane (M) fractions. Δ<i>A</i> Δ<i>L</i> is the Δ<i>dotA</i> Δ<i>dotL</i> double mutant and all of the <i>dotL</i> mutants were expressed in a Δ<i>dotL</i> background. Protein fractions were analyzed by western blot using an IcmS-specific antibody. Fractions were loaded proportionally and are representative of three independent experiments.</p>