Recommended acquisition parameters.

*Promotes hands-free centering of subjects.

**Filling the well to eliminate the meniscus effect (∼340 µL) produced the best results, however, this practice proved impractical for HTS.

+

Promotes maximal throughput and consistently high Z'-factors (Table 2 & 3).

#

Lethality issues were evident at the 400 hertz level when volume was ≤100 µL.