Recombination is increased after THOC1 depletion.
2011-12-01T02:18:09Z (GMT) by
<p>A) Scheme of the pIREC direct-repeat recombination construct used to generate stable HeLa cell lines. B) Recombination analysis using the HeRG stable cell line depleted of THOC1 by siRNA. C) Recombination analysis in the HeTH-4 stable cell lines (−DOX and +DOX) transfected with the plasmid pIREC-direct repeat containing vector. The recombination frequency was measured by FACs analysis as GFP positive cells, 96 h after transfection (B and C). Average and SE from three independent experiments are shown. When the P value of the difference with the siC control calculated with the Mann & Whitney test is <0.05, it is indicated with an asterisk (*).</p>