Figure_2.tif (259.3 kB)
Recombinant plasmid constructs.
figure
posted on 2013-02-23, 11:24 authored by Leticia V. Bentancor, Marcos F. Bilen, María P. Mejías, Romina J. Fernández-Brando, Cecilia A. Panek, Maria V. Ramos, Gabriela C. Fernández, Martín Isturiz, Pablo D. Ghiringhelli, Marina S. PalermopStx2 is the pGEM-T plasmid with the stx2 locus cloned in the same direction that lacZ promoter. pr1-eGFP and pr7-eGFP are reporter plasmids with the region pr1 or pr7 driving eGFP expression, and Δpr-eGFP is a construction lacking the wild CMV promoter and was used as negative control. Linear pr1-eGFP and pr7-eGFP were obtained after digestion with PciI. In pr1ΔTATA-eGFP and pr7ΔTATA-eGFP TATA Box sequence has been replaced by BamHI restriction site. Stx2 linear DNA was obtained after digestion with EcoRI.
