Fig_5.tif (1.18 MB)
Real-time PCR is applied and relative gene expression levels are determined by the comparative Ct method.
figure
posted on 2015-06-03, 03:28 authored by Ryuhei Kanaoka, Akifumi Kushiyama, Yasuyuki Seno, Yusuke Nakatsu, Yasuka Matsunaga, Toshiaki Fukushima, Yoshihiro Tsuchiya, Hideyuki Sakoda, Midori Fujishiro, Takeshi Yamamotoya, Hideaki Kamata, Akio Matsubara, Tomoichiro AsanoBars indicate the means±S.E. of the ratios of the relative expressions of the target mRNAs to that of GAPDH. The mean of the control is arbitrarily set at 1. Statistical significance is indicated by * (p<0.05) or ** (p<0.01). A. LNCap cells were incubated with control solvent (C) or 10 μM Juglone (J) for 48hr. B. DU145 cells were incubated with control solvent (C) or 10 μM Juglone (J) for 48hr.
