Figure_8.tif (142.61 kB)
Re-expression of Pyk2 stimulates migration of miR-23b overexpressing cells.
figure
posted on 2013-02-20, 03:30 authored by Joseph C. Loftus, Julianna T. D. Ross, Kimberly M. Paquette, Vincent M. Paulino, Sara Nasser, Zhongbo Yang, Jean Kloss, Seungchan Kim, Michael E. Berens, Nhan L. TranA. T98G cells transduced with empty vector (ctrl) were left uninfected while T98G cells overexpressing miR-23b were infected with adenoviruses encoding ß-galactosidase, or FLAG epitope-tagged wild-type Pyk2 (Pyk WT) or Pyk2 lacking the 3' UTR (Pyk 3Kb) at MOI = 5. Sixteen hours after infection, cells were plated onto laminin coated slides and the migration rate determined after 24 hours. Values are mean ± SD of 10 replicate samples (*: p<0.05). B. Whole cell lysates were immunoblotted with the indicated antibodies.
