Rarefaction data analysis.
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(A) Left axis: Clustering of OTUs (as % of total OTUs) according to the number of supporting reads. Right axis: % of the total reads included in each category of OTUs. (B) Rarefaction curves at 97% similarity threshold (OTUs/sampling effort) for ITS1 and ITS2 reads. The rarefaction was calculated both with the total pool of OTUs and with only the non-singletons OTUs.