Raptor and rictor are dispensable for translational activation of TOP mRNAs by amino acids.

(A) HEK293 cells were infected with viruses expressing Red shRNA, raptor shRNA (Rap) or rictor shRNAs (Ric). The abundance of raptor or rictor, as well the phosphorylation status of direct and indirect substrates of the respective complexes, mTORC1 and mTORC2 (left and right, respectively), was monitored by Western blot analysis. (B) HEK293 cells infected with viruses expressing HcRed, raptor or rictor shRNA were amino acid starved for 3 h (−AA) or starved and then refed for 3 h (−AA→+AA). Cytoplasmic extracts from these cells were subjected to polysomal analysis and the data are presented as described in the legend to Fig. 2F. Numbers above bars are individual values, when only two measurements were performed.