Figure_1.tif (821.46 kB)
Radiation resistance and genome structure of D. geothermalis and D. radiodurans. A, IR (60Co, 5.5 kGy/h).
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posted on 2007-09-26, 01:21 authored by Kira S. Makarova, Marina V. Omelchenko, Elena K. Gaidamakova, Vera Y. Matrosova, Alexander Vasilenko, Min Zhai, Alla Lapidus, Alex Copeland, Edwin Kim, Miriam Land, Konstantinos Mavromatis, Samuel Pitluck, Paul M. Richardson, Chris Detter, Thomas Brettin, Elizabeth Saunders, Barry Lai, Bruce Ravel, Kenneth M. Kemner, Yuri I. Wolf, Alexander Sorokin, Anna V. Gerasimova, Mikhail S. Gelfand, James K. Fredrickson, Eugene V. Koonin, Michael J. DalyB, UV (254 nm) (3 J/m2 s−1). Open circle, D. radiodurans (32°C); open triangle, D. geothermalis (50°C); and open square, D. geothermalis (32°C). Values are from three independent trials with standard deviations shown. At near-optimal growth temperatures, the 10% survival values (D10) following IR for D. radiodurans (32°C) and D. geothermalis (50°C) are 15 kGy; for E. coli, 0.7 kGy (37°C) [5]; and for T. thermophilus (HB27) 0.8 kGy (65°C) [27]. C, PFGE of genomic DNA prepared from irradiated (0.2 kGy) D. radiodurans (DR+IR) and D. geothermalis (DG+IR); and genomic DNA from non-irradiated D. geothermalis digested with SpeI (DG+SpeI). (M) PFGE DNA size markers. PFGE was as described previously [77].
