RT-qPCR of various regions of the putative CLC locus.

LVS was passed in defined medium and grown on defined medium agar at 32°C in CO₂ to maximize CLC content, or in defined medium broth at 37°C without preliminary passage to minimize CLC. The RNA was isolated, converted to cDNA, and the cDNA amplified by quantitative real-time PCR. The results are shown as the x-fold change in gene expression using LVS grown in BHIC broth to log phase (minimal CLC expression) as the calibrator and GAPDH as the endogenous control for gene expression. The locus tag of each gene from LVS is shown on the X-axis.