RPE cell size and relative abundance of binucleate RPE cells across the mouse and human retina.

(A-C) Confocal images of a flatmount of a BALB/C mouse RPE, showing cells at retinal eccentricities of 0° (A), 40° (B), and 75° (C). Scale bar = 50 μm. (D) Graph of RPE cell cross-sectional area in relation to eccentricity in the mouse and human retinas. RPE cell size was significantly different between eccentricities in both mouse strains (P < 0.0001; Tukey’s test). (E) Graph of RPE cell density in relation to eccentricity in the mouse retinas, illustrating the proportion of binucleate cells, which was different between eccentricities in both mouse strains (P < 0.0001; Tukey’s test). Error bars in D and E indicate SEM. The human data are from Ts’o and Friedman [39].