Figure_5.tif (1.88 MB)
RNA Conformational Changes That Differentiate Ex Virio and In Vitro Transcript States
figure
posted on 2008-04-29, 00:58 authored by Kevin A Wilkinson, Robert J Gorelick, Suzy M Vasa, Nicolas Guex, Alan Rein, David H Mathews, Morgan C Giddings, Kevin M Weeks(A) The in vitro transcript forms a loop-loop dimer. Identical samples of the transcript RNA were resolved in nondenaturing gels, either omitting or containing 7 mM MgCl2. Markers for monomeric (M) and dimeric (D) conformations are shown.
(B) SHAPE reactivity as a function of nucleotide position for ex virio (red) and transcript (black) RNAs. Difference plot in lower panel has been smoothed over a 5-nt window.
(C) Secondary structure model for the primer binding site in the absence of tRNA(lys3).
