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RKIP level modulations alters HT29 but not HCT116 CRC cells' sensitivity to chemotherapeutic agents.

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posted on 2012-01-18, 00:29 authored by Fahd Al-Mulla, Milad S. Bitar, Jingwei Feng, Sungdae Park, Kam C. Yeung

A, HT29 and B, HCT116 cells were stably transfected with different retroviral expression vectors as indicated. Transfected cells were treated with doxorubicin for 48 h before harvesting. Extracts were prepared for western immunoblot analysis with the specific indicated antibodies. C, HCTT116 cells were stably transfected with different retroviral expression vectors as indicated. Transfected cells were treated with 5-FU for 48 h. Extracts were prepared for cytometric analysis to examine binding to 7-ADD and annexin V.

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