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RCAN1.4 induction requires Ca2+, calcineurin and PKC activity.

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posted on 2013-02-21, 00:40 authored by Katherine Holmes, Elinor Chapman, Violaine See, Michael J. Cross

HDMEC were incubated with the inhibitors ZM (3 µM), BAP (20 mM), EGTA (2.5 mM), GFX (3 µM), Go (3 µM). CsA (100 nM) or U0126 (5 µM) for 30 minutes, and then stimulated with: (A) VEGF-A (50 ng/ml), (B) PMA (100 nM), or (C) A23187 (5 µM) for 1 hour. Expression of RCAN1.4 mRNA, relative to unstimulated (basal) cells, was determined by RT-qPCR. Results were normalised to β-actin expression. Data shows mean ± SD (n = 3). **P<0.01, versus VEGF/PMA/A23187 treated. BAP – BAPTA/AM, CsA – cyclosporin A, GFX - GF109203X, Go – Gö6976, ZM – ZM323881.

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