Quantitative PCR of mutant and wildtype hypertrophic zones.

<p>qPCR with primers specific for <i>(A) Agc1</i>, <i>(B) Ctgf</i>, <i>(C) Matn1</i>, <i>(D) Creld2</i>, <i>(E) Derl3</i>, <i>(F) Ero1l</i>, <i>(G) Fgf21</i>, <i>(H) Steap1</i>, and <i>(I) p58IPK</i> on cDNA derived from <i>Wt</i>, <i>Xbp1</i><sup><i>CartΔEx2</i></sup>, <i>ColX</i><sup><i>N617K</i></sup> and <i>C/X</i> hypertrophic zone aRNA. Plots depict mean fold differences with standard deviation from the mean, N = 3, statistical significance was determined using Student’s <i>t</i> test, ** <i>p</i> < 0.01, *** <i>p</i> < 0.001, **** <i>p</i> < 0.0001.</p>