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Quantitative PCR of mutant and wildtype hypertrophic zones.

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posted on 2015-09-15, 03:14 authored by Trevor L. Cameron, Katrina M. Bell, Irma L. Gresshoff, Lisa Sampurno, Lorna Mullan, Joerg Ermann, Laurie H. Glimcher, Raymond P. Boot-Handford, John F. Bateman

qPCR with primers specific for (A) Agc1, (B) Ctgf, (C) Matn1, (D) Creld2, (E) Derl3, (F) Ero1l, (G) Fgf21, (H) Steap1, and (I) p58IPK on cDNA derived from Wt, Xbp1CartΔEx2, ColXN617K and C/X hypertrophic zone aRNA. Plots depict mean fold differences with standard deviation from the mean, N = 3, statistical significance was determined using Student’s t test, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

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    PLOS Genetics

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    Disease Schmid metaphyseal chondrodysplasiacanonical ER stress sensorsXBPexogenous misfolding proteinchopperkhypertrophic zone collagenGADDchondrocyte differentiationhypertrophic chondrocytesupratfCol 10a Mouse models phenocopying MCDSpathologyColXN 617Kgrowth plate cartilage hypertrophic zone expansionhypertrophic zone cartilagehypertrophic zone expansionirerunx

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